Comprehensive study of serratia peptidase immobilization from Serratia sp ZF03 onto chitosan nanogels

作者：Salarizadeh Navvabeh; Hasannia Sadegh^*; Sajedi Reza Hassan; Lamei Navid; Mohsenifar Afshin; Noghabi Kambiz Akbari

来源：Turkish Journal of Biochemistry, 2016, 41(6): 377-383.

DOI：10.1515/tjb-2016-0114

• Serratia peptidase
• Chitosan nanogel
• Enzyme immobilization

Objective: In the present work, we have extended the study and immobilized the metalloprotease enzyme in glutaraldehyde cross-linked chitosan nanogels to scrutinize the enzyme's features including stability over its soluble free form.
Method: The immobilized metalloprotease was characterized using scanning electron microscopy (SEM), followed by Fourier transform infrared (FTIR) spectroscopy. The enzyme is optimally active at 50 degrees C and pH range of 8.0-10.
Results: Thermal stability of the enzyme enhanced when immobilized on the nanogel. After 5 min of incubation at 50 degrees C, immobilized enzymes retained 60% of their original activity, while negligible activity (23%) was observed in the case of the free enzyme.
Conclusion: The results obtained here provide a powerful demonstration of the benefits of taking the glutaraldehyde cross-linked chitosan matrices to enhance metalloprotease stability. The high stability of the immobilized enzyme serves to improve its performance for possible application on the industrial scale.

• 出版日期2016-12