Mouse prickle1, the homolog of a PCP gene, is essential for epiblast apical-basal polarity

作者：Tao Hirotaka; Suzuki Makoto; Kiyonari Hiroshi; Abe Takaya; Sasaoka Toshikuni; Ueno Naoto^*

来源：Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(34): 14426-14431.

DOI：10.1073/pnas.0901332106

• anterior visceral endoderm
• primitive streak
• Vangl2/stbm

Planar cell polarity (PCP) genes are essential for establishing planar cell polarity in both invertebrate and vertebrate tissues and are known to regulate cellular morphogenesis and cell movements during development. We focused on Prickle, one of the core components of the PCP pathway, and deleted one of two mouse prickle homologous genes, mpk1. We found that the deletion of mpk1 gene resulted in early embryonic lethality, between embryonic day (E)5.5 and E6.5, associated with failure of distal visceral endoderm migration and primitive streak formation. The mpk1(-/-) epiblast tissue was disorganized, and analyses at the cellular level revealed abnormal cell shapes, mislocalized extracellular matrix (ECM) proteins, and disrupted orientation of mitotic spindles, from which loss of apico-basal (AB) polarity of epiblast cells are suspected. Furthermore, we show mpk1 genetically interacts with another core PCP gene Vangl2/stbm in the epiblast formation, suggesting that PCP components are commonly required for the establishment and/or the maintenance of epiblast AB polarity. This was further supported by our finding that overexpression of Delta PET/LIM (Delta P/L), a dominant-negative Pk construct, in Xenopus embryo disrupted uniform localization of an apical marker PKC zeta, and expanded the apical domain of ectoderm cells. Our results demonstrate a role for mpk1 in AB polarity formation rather than expected role as a PCP gene.

• 出版日期2009-8-25