X-Ray Dark-field Imaging to Depict Acute Lung Inflammation in Mice

作者：Hellbach Katharina; Meinel Felix G; Conlon Thomas M; Willer Konstantin; Yaroshenko Andre; Velroyen Astrid; Braunagel Margarita; Auweter Sigrid; Reiser Maximilian F; Eickelberg Oliver; Pfeiffer Franz; Yildirim Ali Oe

来源：Scientific Reports, 2018, 8(1): 2096.

DOI：10.1038/s41598-018-20193-8

<jats:title>Abstract</jats:title><jats:p>The aim of this study was to evaluate the feasibility of early stage imaging of acute lung inflammation in mice using grating-based X-ray dark-field imaging <jats:italic>in vivo</jats:italic>. Acute lung inflammation was induced in mice by orotracheal instillation of porcine pancreatic elastase. Control mice received orotracheal instillation of PBS. Mice were imaged immediately before and 1 day after the application of elastase or PBS to assess acute changes in pulmonary structure due to lung inflammation. Subsequently, 6 mice from each group were sacrificed and their lungs were lavaged and explanted for histological analysis. A further 7, 14 and 21 days later the remaining mice were imaged again. All images were acquired with a prototype grating-based small-animal scanner to generate dark-field and transmission radiographs. Lavage confirmed that mice in the experimental group had developed acute lung inflammation one day after administration of elastase. Acute lung inflammation was visible as a striking decrease in signal intensity of the pulmonary parenchyma on dark-field images at day 1. Quantitative analysis confirmed that dark-field signal intensity at day 1 was significantly lower than signal intensities measured at the remaining timepoints, confirming that acute lung inflammation can be depicted <jats:italic>in vivo</jats:italic> with dark-field radiography.</jats:p>

• 出版日期2018-2-1