Alizarin red S-zinc(II) fluorescent ensemble for selective detection of hydrogen sulphide and assay with an H2S donor

作者：Kaushik Rahul; Kumar Pawan; Ghosh Amrita; Gupta Neha; Kaur Davinder; Arora Saroj; Jose D Amilan^*

来源：RSC Advances, 2015, 5(97): 79309-79316.

DOI：10.1039/c5ra11901d

A new Alizarin Red S based fluorescent probe ARS-Zn(II) has been reported for the detection of H2S in aqueous buffer solution. In the presence of zinc ions, ARS displayed an increase in fluorescence intensity through the formation of an ARS-Zn(II) ensemble. The addition of H2S to the ensemble caused the disassembly of the metal and ARS, which restored the fluorescence intensity of ARS. This sensing ensemble exhibited H2S-selectivity over other biothiols and biologically relevant analytes. The calculated detection limit of ARS-Zn(II) with H2S was 92 nM. ARS-Zn(II) also detected H2S in serum under physiological conditions. Moreover, as a practical application, it could be used for the real time monitoring of H2S released from the H2S-donor molecule benzoic (methyl carbonic) dithioperoxyanhydride and also applied to live cell imaging. The reported ensemble ARS-Zn(II) has advantages like ready availability, high selectivity with good sensitivity, fast response time, higher excitation (520 nm) and emission (625 nm) wavelengths and real time fluorescence assay with H2S donors.

• 出版日期2015