A novel amperometric glucose biosensor based on reconstitution of glucose oxidase on thiophene-3-boronic acid polymer layer

作者：Senel Mehmet^*; Dervisevic Muamer; Cevik Emre

来源：Current Applied Physics, 2013, 13(7): 1199-1204.

DOI：10.1016/j.cap.2013.03.004

• Polythiophene
• Glucose oxidase
• Biosensor
• Boronic acid

The biosensor was constructed for determination of glucose by using glucose oxidase enzyme immobilized on poly(thiophene-3-boronic acid) (PTBA). Boronic acid functionalized polythiophene layer was obtained by electrochemical polymerization of Thiophene (Th) and thiophene-3-boronic acid (TBA) with different monomer rations. The reconstitution of the apo-glucose oxidase (apo-GOx) on a complexed flavin adenine dinucleotide (FAD) linked to polythiophene boronic acid (PTBA) monolayer yields an electrically contacted enzyme monolayer. The GOx-reconstituted enzyme electrode exhibited excellent electrocatalytic activities toward the reduction and oxidation of hydrogen peroxide as well. The PTBA/FAD/GOx biosensor shows an excellent performance for glucose at +0.4 V with a high sensitivity (2.14 mu A/mM) and lower response time (similar to 5 s) in a wide concentration range of 0.5-18 mM (correlation coefficient of 0.9952). Furthermore, the effects of applied potential, pH, temperature, electroactive interference, stability and reusability of the biosensors were discussed.

• 出版日期2013-9