Multiwalled-carbon-nanotubes-based matrix solid-phase dispersion extraction coupled with high-performance liquid chromatography for the determination of honokiol and magnolol in Magnoliae Cortex

作者：Zhang Qi; Hong Bo; Liu Jianhua; Mu Guangze; Cong Huan; Li Gang; Cai Defu^*

来源：Journal of Separation Science, 2014, 37(11): 1330-1336.

DOI：10.1002/jssc.201301046

• Box-Behnken design
• Honokiol
• Magnolol
• Matrix solid-phase dispersion
• Response surface methodology

In this paper, multiwalled-carbon-nanotube-based matrix solid-phase dispersion coupled to HPLC with diode array detection was used to extract and determine honokiol and magnolol from Magnoliae Cortex. The extraction efficiency of the multiwalled-carbon-nanotube-based matrix solid-phase dispersion was studied and optimized as a function of the amount of dispersing sorbent, volume of elution solvent, and flow rate of elution solvent, with the aid of response surface methodology. An amount of 0.06 g of carboxyl-modified multiwalled carbon nanotubes and 1.5 mL of methanol at a flow rate of 1.1 mL/min were selected. The method obtained good linearity (r(2) > 0.9992) and precision (RSD < 4.7%) for honokiol and magnolol, with limits of detection of 0.045 and 0.087 mu g/mL, respectively. The recoveries obtained from analyzing in triplicate spiked samples were determined to be from 90.23 to 101.10% and the RSDs from 3.5 to 4.8%. The proposed method that required less samples and reagents was simpler and faster than Soxhlet and maceration extraction methods. The optimized method was applied for analyzing five real samples collected from different cultivated areas.

• 出版日期2014-6
• 单位河北医科大学; 齐齐哈尔医学院