Maternal obesity exacerbates insulitis and type 1 diabetes in non-obese diabetic mice

作者：Wang Hui; Xue Yansong; Wang Baolin; Zhao Junxing; Yan Xu; Huang Yan; Du Min; Zhu Mei Jun^*

来源：Reproduction, 2014, 148(1): 73-79.

DOI：10.1530/REP-13-0614

Accompanying the dramatic increase in maternal obesity, the incidence of type 1 diabetes (T1D) in children is also rapidly increasing. The objective of this study was to explore the effects of maternal obesity on the incidence of T1D in offspring using non-obese diabetic (NOD) mice, a common model for TID. Four-week-old female NOD mice were fed either a control diet (10% energy from fat, CON) or a high-fat diet (60% energy from fat) for 8 weeks before mating. Mice were maintained in their respective diets during pregnancy and lactation. All offspring mice were fed the CON to 16 weeks. Female offspring (16-week-old) born to obese dams showed more severe islet lymphocyte infiltration (major manifestation of insulitis) (P%26lt;0.01), concomitant with elevated nuclear factor kappa-light-chain-enhancer of activated B cells p65 signaling (P%26lt;0.01) and tumor necrosis factor alpha protein level (P%26lt;0.05) in the pancreas. In addition, maternal obesity resulted in impaired (P%26lt;0.05) glucose tolerance and lower (P%26lt;0.05) serum insulin levels in offspring. In conclusion, maternal obesity resulted in exacerbated insulitis and inflammation in the pancreas of NOD offspring mice, providing a possible explanation for the increased incidence of T1D in children.

• 出版日期2014-7