Up-regulation of Idh3 alpha causes reduction of neuronal differentiation in PC12 cells

作者：Cho Sun A; Seo Min Ji; Ko Je Yeong; Shim Jung Hee; Yoo Jin; Kim Jung Hee; Kim Se Yoon; Ryu Na Kyung; Park Eun Young; Lee Han Woong; Lee Yeon Su; Bahk Young Yil^*; Park Jong Hoon

来源：BMB Reports, 2010, 43(5): 369-374.

DOI：10.5483/BMBRep.2010.43.5.369

• Idh3 alpha Neuronal differentiation
• NGF
• PC12 cell
• 2-DE

The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate Idh3 alpha expression by the time course. To identify the function of Idh3 alpha in neuronal differentiation stage, the transfection of Idh3 alpha to PC12 cells was performed. As a result, we proved that up-regulation of Idh3 alpha causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that Idh3 alpha plays a role to the neuronal differentiation. [BMB reports 2010; 43(5): 369-374]

• 出版日期2010-5-31