An In Vivo Transfection Approach Elucidates a Role for Aedes aegypti Thioester-Containing Proteins in Flaviviral Infection

作者：Cheng Gong^*; Liu Lei; Wang Penghua; Zhang Yue; Zhao Yang O; Colpitts Tonya M; Feitosa Fabiana; Anderson John F; Fikrig Erol

来源：PLos One, 2011, 6(7): e22786.

DOI：10.1371/journal.pone.0022786

Mosquitoes transmit pathogens that cause infectious diseases of global importance. Techniques to easily introduce genes into mosquitoes, however, limit investigations of the interaction between microbes and their arthropod vectors. We now show that a cationic liposome significantly enhances delivery and expression of plasmid DNA in Aedes aegypti and Anopheles gambiae mosquitoes. We then introduced the genes for Ae. aegypti thioester-containing proteins (AeTEPs), which are involved in the control of flaviviral infection, into mosquitoes using this technique. In vivo transfection of AeTEP-1 into Ae. aegypti significantly reduced dengue virus infection, suggesting that the approach can further our understanding of pathogen-mosquito interactions.

• 出版日期2011-7-27