Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation

作者：Fogarty Mark C; Hughes Ciara M; Burke George; Brown John C; Davison Gareth W^*

来源：British Journal of Nutrition, 2013, 109(2): 293-301.

DOI：10.1017/S0007114512000992

• Exhaustive exercise
• Watercress prophylaxis
• DNA damage
• Reactive oxygen species

Pharmacological antioxidant vitamins have previously been investigated for a prophylactic effect against exercise-induced oxidative stress. However, large doses are often required and may lead to a state of pro-oxidation and oxidative damage. Watercress contains an array of nutritional compounds such as beta-carotene and alpha-tocopherol which may increase protection against exercise-induced oxidative stress. The present randomised controlled investigation was designed to test the hypothesis that acute (consumption 2 h before exercise) and chronic (8 weeks consumption) watercress supplementation can attenuate exercise-induced oxidative stress. A total of ten apparently healthy male subjects (age 23 (SD 4) years, stature 179 (SD 10) cm and body mass 74 (SD 15) kg) were recruited to complete the 8-week chronic watercress intervention period (and then 8 weeks of control, with no ingestion) of the experiment before crossing over in order to compete the single-dose acute phase (with control, no ingestion). Blood samples were taken at baseline (pre-supplementation), at rest (pre-exercise) and following exercise. Each subject completed an incremental exercise test to volitional exhaustion following chronic and acute watercress supplementation or control. The main findings show an exercise-induced increase in DNA damage and lipid peroxidation over both acute and chronic control supplementation phases (P%26lt;0.05 v. supplementation), while acute and chronic watercress attenuated DNA damage and lipid peroxidation and decreased H2O2 accumulation following exhaustive exercise (P%26lt;0.05 v. control). A marked increase in the main lipid-soluble antioxidants (alpha-tocopherol, gamma-tocopherol and xanthophyll) was observed following watercress supplementation (P%26lt;0.05 v. control) in both experimental phases. These findings suggest that short-and long-term watercress ingestion has potential antioxidant effects against exercise-induced DNA damage and lipid peroxidation.

• 出版日期2013-1-28