Applying AFLPs in Aizoaceae: The Delosperma herbeum complex as a case study

作者：Buys M H^*; van Ren**urg L L Janse; Mienie C M S; Barker N; Burgoyne P M; Mills L; Van Ren**urg L; Hartmann H

来源：Biochemical Systematics and Ecology, 2008, 36(2): 92-100.

DOI：10.1016/j.bse.2007.08.003

• Aizoaceae
• AFLP
• Delosperma
• mesembryanthema
• neighbor-joining analysis
• neighbor-net analysis
• principal coordinate analysis

Amplified fragment length polymorphic (AFLP) markets were used to discriminate between populations of Delosperma herbeum as well as other species of Delosperma occurring in Gauteng and aqjacent provinces in South Africa. A total of 91 individuals representing 30 populations were examined for 158 polymorphic AFLP markers using the EcoR1 and MseI restriction enzymes. Cluster analysis of AFLP marker scores, visualized through Neighbor-Joining and Neighbor-Net trees as well as Principal Coordinate Analysis, provides an effective means of discriminating between different species in the complex. The lack of bootstrap support for Delosperma davyi in the Neighbor-Joining tree and its position in the Principal Coordinate Analysis probably signifies it to be a superfluous name for D. herbeum. The clustering of the other species also suggests that flower colour is not a reliable feature for grouping species in Delosperma. A number of clusters of D. herheum were correlated to a degree with distribution.

• 出版日期2008-2
• 单位西北大学