Determination of 19 Representative Pesticides in Traditional Chinese Medicines by Dispersive Liquid-Liquid Microextraction and Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

作者：Qin, Yao; Chen, Lina; Yang, Xiaojing; Tang, Ying; Li, Senlin; Liu, Chunming^*

来源：Chromatographia, 2016, 79(13-14): 875-884.

DOI：10.1007/s10337-016-3105-1

• Pesticides
• Chinese traditional medicine
• Ultrasonic-assisted dispersive liquid-liquid microextraction
• UHPLC-MS/MS

The objective of this study was to develop an analytical method based on ultrasonic-assisted dispersive liquid-liquid microextraction (DLLME) for the determination of 19 pesticides in Shengmaiyin and its resulting crude drugs Ophiopogon japonicas and Codonopis pilosula. The target compounds were qualitatively and quantitatively studied via ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) operated in multiple reaction-monitoring modes. The UHPLC-MS/MS conditions were optimized to complete chromatographic separation in 9 min. The correlation coefficient was higher than 0.993 for each pesticide. Average recoveries of the 19 compounds from different fortified samples ranged between 81 and 108 % with inter-RSD values below 13 %. The limits of quantification (LOQs) for 19 compounds were in the range of 0.0075-0.81 mu g kg(-1). Recovery LOQs of the DLLME method were compared with the routine QuEChERS method, and the results showed that the proposed method is very simple, rapid, and sensitive. This makes it a more applicable method for monitoring multiple classes of pesticides in traditional Chinese medicine.

• 出版日期2016-7
• 单位长春师范大学