AN RNA STEM-LOOP STRUCTURE INVOLVED IN THE PACKAGING OF BOVINE LEUKEMIA-VIRUS GENOMIC RNA IN-VIVO

作者：KURG A^*; SOMMER G; METSPALU A

来源：Virology, 1995, 211(2): 434-442.

DOI：10.1006/viro.1995.1425

An RNA secondary structure of the bovine leukemia virus (BLV) 5'-terminal RNA sequence was constructed by computer-assisted RNA secondary structure analysis. Mutations were created in the noncoding region (NCR) of BLV, which contains a conserved consensus sequence, to disrupt predicted secondary structure of this region. After transfection of these constructs into FLK-BLV cells and analysis oi viral particles a reduction in mutant RNA content was observed relative to that of unmutated vector RNA. The packaging efficiency of the mutant with a substitution in the consensus sequence was reduced threefold and that of the mutant with a deleted 5' NCR was reduced fivefold. We conclude that predicted RNA secondary structure and/or nucleotide sequence of the BLV noncoding region is essential for BLV RNA packaging in vivo.

• 出版日期1995-8-20