Deinococcus phoenicis sp nov., an extreme ionizing-radiation-resistant bacterium isolated from the Phoenix Lander assembly facility

作者：Vaishampayan Parag^*; Roberts Anne Hayden; Augustus Angela; Pukall Ruediger; Schumann Peter; Schwendner Petra; Mayilraj Shanmugam; Salmassi Tina; Venkateswaran Kasthuri

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 10): 3441-3446.

DOI：10.1099/ijs.0.063107-0

A bacterial strain, designated 1P10ME(T), which was resistant to extreme doses of ionizing radiation, pale-pink, non-motile, and a tetrad-forming coccoid was isolated from a cleanroom at the Kennedy Space Center, where the Phoenix spacecraft was assembled. Strain 1P10ME(T) showed optimum growth at 30 degrees C, with a pH range for growth of 6.5-9.0 and was highly sensitive to sodium chloride, growing only in medium with no added NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1P10ME(T) represents a novel member of the genus Deinococcus, with low sequence similarities (<93.5 %) to recognized species of the genus Deinococcus. The predominant cellular fatty acid was C-15 : 1 omega 6c. This novel strain exhibits extreme resistance to gamma radiation (D-10 >8 kGy) and UV (D-10 >1000 Jm(-2)). The results of our polyphasic taxonomic analyses suggest that strain 1P10ME(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus phoenicis sp. nov. is proposed. The type strain is 1P10ME(T) (=NRRL B-59546(T)=DSM 27173(T)).

• 出版日期2014-10