Gas Chromatographic Determination of alpha-Keto Acids in Pharmaceutical Preparation Using 1,2-Propylenediamine as Derivatizing Reagent

作者：Abbasi K U; Khuhawar M Y^*; Mahar K P; Mallah Arfana; Siyal A Nawaz

来源：Asian Journal of Chemistry, 2013, 25(3): 1211-1215.

• Gas Chromatography
• alpha-Keto acids
• 1,2-Propylenediamine
• Drug analysis

Analytical procedure has been developed for the gas chromatographic separation and determination of alpha-keto acids: pyruvic acid, 2-ketobutyric acid, 3-methyl-2-oxobutyric acid, 3-methyl-2-oxovaleric acid, 4-methyl-2-oxovaleric acid, 2-ketohexanoic acid and phenylpyruvic acid after derivatization with 1,2-propylenediamine from the column HP-5 (30 x 0.32 mm i.d). The detection was made by flame ionization. Linear calibration curves were obtained within 9.0-84 mu g/mL and detection limits within 3.0-5.6 mu g/mL of each alpha-keto acids. A pharmaceutical preparation ketosteril was analyzed for phenylpyruvic acid, 3-methyl-2-oxovaleric acid, 4-methyl-2-oxovaleric acid and 3-methyl-2-oxobutyric acid and the found amounts correlated with the labeled values. The extraction recovery from pharmaceutical preparation tested by standard addition of these compounds was calculated to be 94.0-96.8 % with RSD 0.1-0.8 %. A number of pharmaceutical additives and amino acids added did not affect the determination of alpha-keto acids with relative error within +/- 3 %.

• 出版日期2013-2