Maternal vitamin D intake during pregnancy increases gene expression of ILT3 and ILT4 in cord blood

作者：Rochat M K^*; Ege M J; Plabst D; Steinle J; Bitter S; Braun Fahrlaender C; Dalphin J C; Riedler J; Roponen M; Hirvonen M R; Buechele G; Renz H; Lauener R; Krauss Etschmann S; von Mutius E

来源：Clinical and Experimental Allergy, 2010, 40(5): 786-794.

DOI：10.1111/j.1365-2222.2009.03428.x

• atopic disease
• birth cohort
• farming environment
• ILT3 and ILT4
• vitamin D

P>Background
Recent studies indicate that prenatal vitamin D intake may protect against the development of atopic diseases in young children. Vitamin D has been shown to induce tolerogenic antigen-presenting cells such as dendritic cells. Whether the allergy-protective potential of prenatal vitamin D is mediated through such mechanisms is, however, unknown.
Objective
To evaluate the association between prenatal vitamin D supplementation and tolerogenic antigen-presenting cells in cord blood (CB) as determined by mRNA measurement of immunoglobulin-like transcripts (ILT)3 and ILT4.
Methods
A prospective multi-centre birth cohort was established in rural areas of five European countries. Information on maternal exposures including vitamin D intake was collected by questionnaires during pregnancy. The gene expression of ILT3 and ILT4 was analysed by real-time PCR in the CB of 927 children. Maternal vitamin D supplementation was assessed in Finland and France (n=349).
Results
Maternal vitamin D supplementation during pregnancy was associated with an increase in the gene expression of ILT3 (P=0.012) and ILT4 (P < 0.001). This association remained significant for ILT4 (P=0.020) and showed a positive trend for the gene expression of ILT3 (P=0.059) after multivariate analysis controlling for various confounders.
Conclusions
Vitamin D supplementation during pregnancy may increase the mRNA levels of ILT3 and ILT4 in CB. This finding may point towards an early induction of tolerogenic immune responses by maternal vitamin D intake.
Cite this as: M. K. Rochat, M. J. Ege, D. Plabst, J. Steinle, S. Bitter, C. Braun-Fahrlander, J-C. Dalphin, J. Riedler, M. Roponen, M-R. Hirvonen, G. Buchele, H. Renz, R. Lauener, S. Krauss-Etschmann, E. von Mutius and the PASTURE Study group, Clinical & Experimental Allergy, 2010 (40) 786-794.

• 出版日期2010-5