A Monoclonal Antibody Against Human UL16-binding Protein 3

作者：Mao, Chaoming^*; Qin, Wei; Chen, Deyu; Yuan, Guoyue; Mou, Xiao; Bao, Danxia; Wu, Wei; Xu, Huaxi; Wang, Shengjun

来源：Hybridoma, 2012, 31(3): 203-208.

DOI：10.1089/hyb.2011.0113

• T-CELL-RECEPTOR
• MALIGNANT DISEASES
• CUTTING EDGE
• SOLUBLE MICA
• NKG2D
• CYTOTOXICITY
• ENGAGEMENT
• EXPRESSION
• COSTIMULATION
• ACTIVATION

The UL16-binding proteins (ULBPs) are a novel family of human MHC class I-related, cell surface proteins that function as ligands for NKG2D. In this study, the gene encoding human ULBP3 was cloned into prokaryotic expression vector pQE30, resulting in a recombinant plasmid pQE30-ULBP3. The pQE30-ULBP3 was transformed into Escherichia coli M15 and induced the expression of recombinant protein ULBP3 (rec-ULBP3). The purified rec-ULBP3 as an antigen was used to immunize BALB/c mice. Through cell fusion, sub-cloning, and screening approach, three hybridoma cell clones expressing monoclonal antibodies (MAb) were acquired. The results from Western blot analysis, flow cytometry, and enzyme-linked immunosorbent assay showed that the hybridoma clones B2-F1-F1 and B4-C5-D11, and not G2-A4-A12, reacted with rec-ULBP3 and nature ULBP3 expressed on the cell surface of the tumor cells. In conclusion, the new MAb described here provides a valuable tool for further investigating ULBP3 function and clinical application.

• 出版日期2012-6
• 单位江苏大学