Nanoflake-like SnS2 matrix for glucose biosensing based on direct electrochemistry of glucose oxidase

作者：Yang, Zhanjun; Ren, Yanyan; Zhang, Yongcai; Li, Juan; Li, Hongbo; Hu, Xiaochun Huang Xiaoya^*; Xu, Qin

来源：Biosensors and Bioelectronics, 2011, 26(11): 4337-4341.

DOI：10.1016/j.bios.2011.04.031

• Nanoflake-like SnS2
• Glucose oxidase
• Direct electrochemistry
• Glucose
• Biosensor

A novel biosensor is developed based on immobilization of proteins on nanoflake-like SnS2 modified glass carbon electrode (GCE). With glucose oxidase (GOD) as a model, direct electrochemistry of the GOD/nanoflake-like SnS2 is studied. The prepared SnS2 has large surface area and can offer favorable microenvironment for facilitating the electron transfer between protein and electrode surface. The properties of GOD/SnS2 are characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV), respectively. The immobilized enzyme on nanoflake-like SnS2 retains its native structure and bioactivity and exhibits a surface-controlled, reversible two-proton and two-electron transfer reaction with the apparent electron transfer rate constant (k(s)) of 3.68 s(-1). The proposed biosensor shows fast amperometric response (8 s) to glucose with a wide linear range from 2.5 x 10(-5) M to 1.1 x 10(-3) M, a low detection limit of 1.0 x 10(-5) M at signal-to-noise of 3 and good sensitivity (7.6 +/- 0.5 mA M-1 cm(-2)). The resulting biosensor has acceptable operational stability, good reproducibility and excellent selectivity and can be successfully applied in the reagentless glucose sensing at -0.45 V. It should be worthwhile noting that it opens a new avenue for fabricating excellent electrochemical biosensor.

• 出版日期2011-7-15
• 单位扬州大学