Antimicrobial and antiviral activity-guided fractionation from Scutia buxifolia Reissek extracts

作者：Boligon Aline Augusti^*; Kubica Thais Felli; Mario Debora Nunes; de Brum Thiele Faccim; Piana Mariana; Weiblen Rudi; Lovato Luciane; Alves Sydney Hartz; Vianna Santos Roberto Christ; dos Santos Alves Camilla Filippi; Athayde Margareth Linde

来源：Acta Physiologiae Plantarum, 2013, 35(7): 2229-2239.

DOI：10.1007/s11738-013-1259-0

• Scutia buxifolia
• Antimicrobial activity
• Biofilm
• HSV-1
• HPLC

Antimicrobial and antiviral activities of the fractions from Scutia buxifolia stem bark and leaves were evaluated. Best antimicrobial results occurred with the ethyl acetate (EA) and n-butanolic (NB) fractions from the leaves against Micrococcus sp. (minimal inhibitory concentration-MIC = 62.5 mu g/ml), and NB fraction from stem bark and leaves against Klebsiella pneumoniae and Enterococcus faecalis (MIC = 62.5 mu g/ml). The most active fractions were selected and fractioned into silica column to perform an in vitro antibiofilm assay, which evidenced subfractions EA2 and EA3 as the more active against Candida albicans (biofilm inhibitory concentration-BIC = 582 +/- A 0.01 mu g/ml) and Staphylococcus aureus (BIC = 360 +/- A 0.007 mu g/ml), respectively. The NB (selectivity index-SI = 25.78) and the EA (SI = 15.97) fractions from the stem bark, and the EA (SI = 14.13) fraction from the leaves exhibited a potential antiviral activity towards Herpes Simplex Virus type 1 whereas EA2 and EA3 subfractions from leaves (SI = 12.59 and 10.06, respectively), and NB2 subfraction from stem bark (SI = 12.34) maintained this good activity. Phenolic acids and flavonoids (gallic acid, chlorogenic acid, caffeic acid, rutin, isoquercitrin, quercitrin and quercetin) were identified by HPLC and may be partially responsible for the antimicrobial and antiherpes activities observed. The results obtained in this study showed that Scutia buxifolia has antibiofilm and anti-herpetic activities and that these properties are reported for the first time for this species.

• 出版日期2013-7