Proteome analysis of waito-c rice seedlings treated with culture fluid of gibberellin-producing fungus, Fusarium proliferatum KGL0401

作者：Rim Soon Ok; Lee Jin Hyung; Hwang Seon Kap; Suh Seok Jong; Lee Jin Man; Rhee In Koo; Kim Jong Guk^*

来源：Journal of Microbiology and Biotechnology, 2006, 16(12): 1990-1994.

• gibberellin
• Fusarium proliferatum KGL0401
• matrix-assisted laser desorption-ionization time-of-flight mass
• spectrometry
• two-dimensional polyacrylamide gel electrophoresis

Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited a high GA productivity. A gas chromatography-mass spectrometry (GC-MS) analysis of extracts of the culture fluid of F proliferatum KGL0401 also revealed the presence of GA(1), GA(3), GA(4), GA(7), GA(20), and GA(24). Therefore, the present study conducted a proteome analysis of waito-c rice treated with the culture fluid of the isolated F proliferatum KGL0401 to identify the protein expression triggered by the GA-containing culture fluid. The results revealed the overexpression of 180 protein spots in the sample treated with the culture fluid. Among them, 75 induced proteins were selected and analyzed by MALDI-TOF (matrix-assisted laser desorption-ionization time-of-flight) mass spectrometry, followed by database searching, and 51 proteins were identified.

• 出版日期2006-12