Anti-inflammatory effects of calcium citrate in RAW 264.7 cells via suppression of NF-kappa B activation

作者：Choi Eun Young; Kim Hak Ju; Han Ji Sook^*

来源：Environmental Toxicology and Pharmacology, 2015, 39(1): 27-34.

DOI：10.1016/j.etap.2014.11.002

• Calcium citrate
• Anti-inflammation
• RAW 264.7 cells

Here we aimed to investigate the anti-inflammatory effects of calcium citrate in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The anti-inflammatory effects of calcium citrate were investigated by assessing pro-inflammatory factors (NO, ROS, NF-kappa B, iNOS, and COX-2) and pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha). Treatment of cells with calcium citrate (10-100 mu M) significantly reduced the generation of intracellular reactive oxygen species and increased the activities of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase in LPS-stimulated macrophages. Calcium citrate was further shown to inhibit NO production in LPS-stimulated RAW 264.7 cells. The expression levels of iNOS, COX-2, and NF-kappa B were also suppressed by treatment with calcium citrate. Calcium citrate was furthermore found to significantly inhibit the production of IL-1 beta, IL-6, and TNF-alpha in response to LPS-stimulation. These findings demonstrate that calcium citrate may be an effective anti-inflammatory agent.

• 出版日期2015-1