Fasciola hepatica tegumental antigens indirectly induce an M2 macrophage-like phenotype in vivo

作者：Adams P N; Aldridge A; Vukman K V; Donnelly S; O' Neill S M^*

来源：Parasite Immunology, 2014, 36(10): 531-539.

DOI：10.1111/pim.12127

• dendritic cells
• eosinophils
• Fasciola hepatica
• IL-13
• M2 macrophages
• STAT6

The M2 subset of macrophages has a critical role to play in host tissue repair, tissue fibrosis and modulation of adaptive immunity during helminth infection. Infection with the helminth, Fasciola hepatica, is associated with M2 macrophages in its mammalian host, and this response is mimicked by its excretory-secretory products (FhES). The tegumental coat of F.hepatica (FhTeg) is another major source of immune-modulatory molecules; we have previously shown that FhTeg can modulate the activity of both dendritic cells and mast cells inhibiting their ability to prime a Th1 immune response. Here, we report that FhTeg does not induce Th2 immune responses but can induce M2-like phenotype in vivo that modulates cytokine production from CD4(+) cells in response to anti-CD3 stimulation. FhTeg induces a RELM expressing macrophage population in vitro, while in vivo, the expression of Arg1 and Ym-1/2 but not RELM in FhTeg-stimulated macrophages was STAT6 dependent. To support this finding, FhTeg induces RELM expression in vivo prior to the induction of IL-13. FhTeg can induce IL-13-producing peritoneal macrophages following intraperitoneal injection This study highlights the important role of FhTeg as an immune-modulatory source during F.hepatica infection and sheds further light on helminth-macrophage interactions.

• 出版日期2014-10