A Sensitive Electrochemical Method for Detection of Histone Deacetylase with the Signal Amplification of Ferrocene-Capped Gold Nanoparticle/Streptavidin Conjugates

作者：Gao Yanping^*; Sun Ting; Yang Chengye; Zhou Binbin

来源：International Journal of Electrochemical Science, 2015, 10(4): 3230-3235.

• Histone deacetylases
• Electrochemical biosensors
• Gold nanoparticles
• Signal amplification
• Ferrocene

This work reported the amplified voltammetric detection of histone deacetylase (HDAC) using ferrocene (Fc)-capped gold nanoparticle/streptavidin (SA-AuNPs) conjugates. The acetylated peptide substrates immobilized on the gold electrodes were deacetylated by HDAC, resulting in the appearance of free amine groups on electrode surface. The exposed primary amines were then derivatized with biotin for the attachment of Fc-capped SA-AuNPs conjugates. The voltammetric responses were found to be proportional to the HDAC concentrations ranging from 0.1 to 30 nM with a detection limit of 20 pM.

• 出版日期2015-4
• 单位安阳师范学院