Discovery of selective 2,4-diaminopyrimidine-based photoaffinity probes for glyoxalase I

作者：Zhou Yiqing; Guo Tianlin; Li Xitao; Dong Yi; Galatsis Paul; Johnson Douglas S; Pan Zhengying^*

来源：Medchemcomm, 2014, 5(3): 352-357.

DOI：10.1039/c3md00286a

Glyoxalase I (GLO-1) plays a critical role in the detoxification of 2-oxoaldehydes and is highly expressed in cancer cells. Through photo-affinity labelling and affinity pull-down approaches, a series of 2,4-diaminopyrimidine compounds were discovered to selectively bind to GLO-1 in cells. These compounds show potent inhibition of GLO-1 enzyme activity and prevent proliferation of cancer cells. The cell permeable and "clickable" photoaffinity probe L1-Bpyne presented here could be a valuable tool for profiling GLO-1 in live cells.

• 出版日期2014-3
• 单位北京大学