Dipeptidyl peptidase-4 (DPP-4): Localization and activity in human and rodent islets

作者：Liu, Liehua; Omar, Bilal; Marchetti, Piero; Ahren, Bo^*

来源：Biochemical and Biophysical Research Communications, 2014, 453(3): 398-404.

DOI：10.1016/j.bbrc.2014.09.096

• Dipeptidyl peptidase 4
• Incretin
• Islet of Langerhans
• Type 2 diabetes

Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in alpha-cells in human and pig islets, but primarily in beta-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and inhibition of DPP-4 enhanced insulin secretion in the presence of glucagon-like peptide-1 (GLP-1) in the cells. DPP-4 activity was remarkably robust when cultured with high glucose, incretin hormones, and insulin in mouse and human islets as well as INS-1 832/13 cells and islet DPP-4 activity and expression pattern was not altered in double incretin receptor knockout mice, compared to wild type mice. We conclude that islet DPP-4 is species-specifically expressed in alpha-cell and beta-cell dominant patterns in several species and both patterns remained robust in enzyme activity during short-term metabolic challenge.

• 出版日期2014-10-24
• 单位中山大学