Age-and sun exposure-dependent differences in 8-hydroxy-2 '-deoxyguanosine and N-epsilon-(carboxymethyl)lysine in human epidermis

作者：Toyokuni Shinya^*; Hirao Ayaka; Wada Tamae; Nagai Ryoji; Date Akira; Yoshii Takashi; Akatsuka Shinya; Yamashita Yoriko; Kawada Akira

来源：Journal of Clinical Biochemistry and Nutrition, 2011, 49(2): 121-124.

DOI：10.3164/jcbn.11-05

• skin aging
• UV
• oxidative stress
• advanced glycation end product

Aging and exposure to sunlight are two major factors in the deterioration of skin function. In this study, thirty-six fixed human skin samples from sun-exposed and unexposed areas from young and old individuals were used to evaluate the localization of oxidative stress according to levels and distribution of 8-hydroxy-2'-deoxyguanosine and N-epsilon-(carboxymethyl)lysine in samples using immunohistochemistry. In the epidermis of the young, negligible amounts of 8-hydroxy-2'-deoxyguanosine and N-epsilon-(carboxymethyl)lysine were detected in unexposed areas, whereas nuclear 8-hydroxy-2'-deoxyguanosine and cytoplasmic N-epsilon-(carboxymethyl)lysine were increased in the lower epidermis in sun-exposed areas. In contrast, the aged presented prominent nuclear 8-hydroxy-2'-deoxyguanosine and nuclear N-epsilon-(carboxymethyl)lysine in the epidermis of unexposed areas, concomitant with dermal increase in N-epsilon-(carboxymethyl)lysine. However, the immunostaining of 8-hydroxy-2'-deoxyguanosine and N-epsilon-(carboxymethyl)lysine revealed a decrease in the epidermis of sun-exposed areas in the aged. These results suggest an age-dependent difference in the adaptation and protective mechanisms of the epidermis against sunlight-associated oxidative stress, thus necessitating distinct standards for evaluation in each age group. Further investigation is warranted to elucidate underlying molecular mechanisms.

• 出版日期2011-9-1