A High Voltage Power Supply That Mitigates Current Reversals in Capillary Zone Electrophoresis-Electrospray Mass Spectrometry

作者：Flaherty Ryan J; Sarver Scott A; Sun Liangliang; Brownell Greg A; Go David B; Dovichi Norman J^*

来源：Journal of the American Society for Mass Spectrometry, 2017, 28(2): 247-252.

DOI：10.1007/s13361-016-1529-3

• Capillary zone electrophoresis
• Electrospray power supply

Capillary electrophoresis coupled with electrospray ionization typically employs two power supplies, one at each end of the capillary. One power supply is located at the proximal (injection) end of the capillary. The power supply located at the distal (detector) end of the capillary drives the electrospray. Electrophoresis is driven by the difference in potential between these power supplies. Separations that employ large capillary inner diameter, high conductivity background electrolyte, and high separation potentials generate higher current than that produced by the electrospray. Excess current flows through the electrospray power supply. Most power supplies are not designed to sink current, and the excess current will cause the electrospray voltage to deviate from its set point. We report a simple circuit to handle this excess current, allowing separations under a wide range of electrophoretic conditions.

• 出版日期2017-2