A system for the analysis of BKV non-coding control regions: Application to clinical isolates from an HIV/AIDS patient

作者：Broekema Nicole M; Abend Johanna R; Bennett Shauna M; Butel Janet S; Vanchiere John A; Imperiale Michael J^*

来源：Virology, 2010, 407(2): 368-373.

DOI：10.1016/j.virol.2010.08.032

• BKV
• NCCR
• HIV/AIDS

The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BIN exists as archetype virus and rearranged variants, which are classified based on the DNA sequence of their non-coding control regions (NCCRs). The archetype BKV NCCR is divided into five blocks of sequence and rearranged variants contain deletions and duplications of these blocks. In this study, a genetic system was developed and used to identify the major determinant of replication ability in primary renal proximal tubule epithelial cells, the natural host cell of BKV. This system was also used to analyze NCCR variants isolated from an immunocompromised patient which contain assorted rearrangement patterns and functional differences. This study solidifies the NCCR as the major genetic determinant of BIN replication ability in vitro.

• 出版日期2010-11-25