Molecular characterization of a CpTRIM35-like protein and its splice variants from whitespotted bamboo shark (Chiloscyllium plagiosum)

作者：Zhang, Xinshang; Zhao, Heng^*; Chen, Yeyu; Luo, Huiying; Yao, Bin

来源：Biochemical and Biophysical Research Communications, 2014, 453(3): 425-431.

DOI：10.1016/j.bbrc.2014.09.139

• Whitespotted bamboo shark (Chiloscyllium plagiosum Bennett)
• TRIM35-like gene
• Splice variant
• Expression pattern
• Subcellular localization
• Self-ubiquitylation

The tripartite motif (TRIM) proteins play important roles in a broad range of biological processes, including apoptosis, cell proliferation and innate immunity response. In this study, a TRIM gene and its three splice variants were cloned from an elasmobranch fish-whitespotted bamboo shark (Chiloscyllium plagiosum Bennett). Phylogenetic analysis indicated that the gene was closely related to TRIM35 homologs, thus termed CpTRIM35-like. Deduced CpTRIM35 has a RBCC-PRY/SPRY structure typical of TRIM proteins, and its splice variants (CpTRIM35-1-3) have different truncations at the C-terminus. The gene products were constitutively expressed in adult sharks with the highest levels in spleen and kidney. The different subcellular locations, upregulation upon LPS and poly I:C stimulation, and significant E3 ubiquitin ligase activities suggested their different roles in immune responses as an E3 ubiquitin ligase. This is the first TRIM protein ever characterized in elasmobranch fish.

• 出版日期2014-10-24
• 单位中国农业科学院