Aneugenic Effects of Epirubicin in Somatic and Germinal Cells of Male Mice

作者：Attia Sabry Mohamed^*; Ahmad Sheikh Fayaz; Okash Radwa Mohamed; Bakheet Saleh Abdulrahman

来源：PLos One, 2014, 9(10): e109942.

DOI：10.1371/journal.pone.0109942

The ability of the antineoplastic agent epiruibicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo by using the 48 h. Incorporation assay, it could be shown that the meiotic delay caused by epiru ion in germ cells was approximately 48 h. Disornic and diploid sperm were shown in epididyrnal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XYperm sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise he development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical onnel exposed to epirubicin

• 出版日期2014-10-10