Acute toxicity and long-term safety evaluation of the crude extract from rhizomes of Limonium brasiliense in mice and rats

作者：Antonelli Ushirobira Tania M; Blainski Andressa; Fernandes Henrique G; Moura Costa Gislaine F; Costa Marco A; Campos Shimada Lilian B; Salgueiro Pagadigorria Clairce L; Kaneshima Edilson N; Becker Tania C A; Leite Mello Eneri V S; de Mello Joao C P^*

来源：Journal of Ethnopharmacology, 2015, 174: 293-298.

DOI：10.1016/j.jep.2015.08.022

• Limonium brasiliense
• Preclinical toxicity
• Redox state of the liver
• Mutagenicity
• Plumbaginaceae

Ethnopharmacological relevance: Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, popularly known as baicuru, has been used in folk medicine to treat menstrual cramps and to regulate menstrual periods. However, little is known about its safety. This study evaluated the safety through in vivo tests of the acute, long-term, and liver toxicity, and the mutagenicity of the crude extract (CE) from rhizomes of L. brasiliense.
Materials and methods: The acute toxicity was assessed in Swiss mice, and the chronic toxicity in Wistar rats. Male and female mice received the CE orally in single doses of 1.0, 2.0, 3.0, 4.0, or 5.0 g/kg. Clinical changes and mortality rate were used as parameters to assess the acute toxicity. In the long-term evaluation, male and female Wistar rats were treated orally with daily doses of the CE (50, 100, or 200 mg/kg) for 90 days. Assessments of weight, behavior and food intake, urinalysis, biochemical and hematological analyses, as well as macro- and microscopic observations of several organs were performed. The redox state of the liver was evaluated as a means of investigating the liver toxicity, and the micronucleus test to assess mutagenicity was also performed.
Results: Evaluation of acute toxicity indicated no apparent clinical change in the animals; the LD50 was 4.8 g/kg. Evaluation after 90 days administration showed that the CE, even in higher doses than are considered therapeutic, appeared to be safe. The micronucleus test demonstrated a low mutagenic potential for the CE.
Conclusion: Our results showed that treatment with the CE from L. brasiliense caused low or no toxicity, as assessed using these doses and evaluation methods.

• 出版日期2015-11-4