Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp chinensis var. purpurea)

作者：Guo, Ning; Wu, Jian; Zheng, Shuning; Cheng, Feng; Liu, Bo; Liang, Jianli; Cui, Yang; Wang, Xiaowu^*

来源：Molecular Breeding, 2015, 35(5): 113.

DOI：10.1007/s11032-015-0237-1

• Anthocyanin profile
• QTL
• EGL3
• Brassica rapa

Anthocyanins have several biological functions in plants and are beneficial to human health. To elucidate the metabolic profile of anthocyanins and determine the genetic basis controlling anthocyanin accumulation in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea), we conducted anthocyanin profile characterization and quantitative trait locus (QTL) analysis. Seventeen anthocyanin compounds were identified as cyanidin glycosides in zicaitai. A genetic linkage map based on 200 F2 lines was constructed using 161 insertion/deletion markers. Total anthocyanin content (TAC) was determined by pH differential spectrophotometry for the F2 lines. Using the map and phenotypic data, a major QTL which explained 56.7 % of phenotypic variation was identified for TAC on chromosome A09. Two genes, BrEGL3.1 and BrEGL3.2, as syntenic orthologs of AtEGL3 encoding basic helix-loop-helix transcription factors in this QTL region, are candidate genes for a key role in the control of anthocyanin accumulation in zicaitai.

• 出版日期2015-5
• 单位中国农业科学院