A novel translational model for fetoscopic intratracheal delivery of nanoparticles in piglets

作者：Carlon Marianne S; Engels Alexander C; Bosch Barbara; Joyeux Luc; Mori da Cunha Marina G M C; Vidovic Dragana; Debyser Zeger; De Boeck Kris; Neyrinck Arne; Deprest Jan A^*

来源：Prenatal Diagnosis, 2016, 36(10): 926-934.

DOI：10.1002/pd.4915

ObjectivesThe aim of this study was to assess the feasibility of fetal tracheal injection in the late-gestational pig to target the airways.
MethodsFollowing laparotomy and hysterotomy, fetoscopy was performed in pregnant sows to access the fetal trachea. Two volumes of fluospheres were injected (1 and 3mL). Fluosphere distribution to the different lung lobes was investigated by microscopy. Possible fetal airway injury, caused by the surgical procedure or intratracheal injection, was investigated. Lung morphology and fetal lung volumes were calculated by micro computed tomography (CT).
ResultsIntratracheal administration was successfully performed in 20/21 fetuses. Analysis by confocal microscopy demonstrated that 3mL, and not 1mL, most efficiently targeted all lung lobes. On high-resolution CT, total airway volume was estimated at 2.9mL; strengthening that 3mL is appropriate to target all lung lobes. No procedural damage was evidenced in the lungs or trachea.
ConclusionsIntratracheal injection of nanoparticles is feasible in the pregnant pig and does not cause procedural lung damage. Using an injection volume of 3mL, all lung lobes were efficiently targeted. This nanoparticle delivery model to fetal airways opens perspectives for therapeutic interventions.

• 出版日期2016-10