Application of aurintricarboxylic acid for the adherence of mouse P19 neurons and primary hippocampal neurons to noncoated surface in serum-free culture

作者：Lee Tsung Yih; Chen Wen Shin; Huang Yung An; Liu Ting Wei; Hwang Eric; Tseng Ching Ping^*

来源：Biotechnology Progress, 2012, 28(6): 1566-1574.

DOI：10.1002/btpr.1638

• aurintricarboxylic acid
• serum-free medium
• P19 neuron cells
• hippocampal neurons
• cell attachment reagent

Dissociated primary neuron culture has been the most widely used model systems for neuroscience research. Most of these primary neurons are cultured on adhesion matrix-coated surface to provide a proper environment for cell anchorage under serum-free conditions. In this study, we provide an alternative technique to promote the adhesions of these neurons using aurintricarboxylic acid (ATA), a nonpeptide compound, without surface manipulations. We first demonstrated that ATA could promote Chinese hamster ovary cell attachment and proliferation in serum-free medium in a dosage-dependent manner. We later showed that ATA significantly enhanced the attachment of the retinoic acid differentiated P19 mouse embryonal carcinoma (P19) neurons, with an optimal concentration around 30 mu g/mL. A similar result was seen in primary hippocampal neurons, with an optimal ATA concentration around 15 mu g/mL. Further morphological assessments revealed that the average neurite length and neuronal polarization were almost identical to that obtained using a conventional method with poly-L-lysine surface. The advantages of using the ATA treatment technique for immunochemical analysis are discussed.

• 出版日期2012-12