A plant-tissue-based biophotonic method for herbicide sensing

作者：Guo Ya; Tan Jinglu^*

来源：Biosensors and Bioelectronics, 2010, 25(8): 1958-1962.

DOI：10.1016/j.bios.2010.01.022

• DCMU
• Delayed fluorescence
• Herbicide

Herbicide application incurs water contamination and thus effective herbicide detection methods are needed. In this research, a kinetic model was developed to describe the processes of herbicide diffusion into plant tissues and binding to the active sites. DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea], a commonly used herbicide, was used as a test chemical and its diffusion into plant leaves and binding to plastoquinone B (Q(B)) sites were analyzed by using the model. The dependency of delayed fluorescence (DF) emission on unbound Q(B) sites was first derived theoretically and then observed experimentally. This yields a biophotonic method to measure the concentrations of DCMU and other herbicides that function by a similar mechanism. Experiments were performed to validate the developed method.

• 出版日期2010-4-15