A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

作者：Castro Andre L^*; Tarelho Sonia; Dias Mario; Reis Flavio; Teixeira Helena M

来源：Journal of Pharmaceutical and Biomedical Analysis, 2016, 119: 139-144.

DOI：10.1016/j.jpba.2015.11.038

• Forensic science
• Gamma-hydroxybutyric acid (GHB)
• GC-MS/MS
• Analytical validation
• Endogenous values
• Forensic toxicology

Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market.
A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100 mu L of whole blood, obtained results included a LOD and LLOQ of 0.1 mg/L and a recovery of 86% in a working range between 0.1 and 100 mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D-6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

• 出版日期2016-2-5