Arsenic processing of yeast isolates IIB-As1 & IIB-As2 and production of glutathione under stress conditions

作者：Muneer Bushra^*; Lali Tayyaba; Iqbal Muhammad J; Shakoori Farah R; Shakoori Abdul R

来源：Journal of Basic Microbiology, 2016, 56(10): 1124-1131.

DOI：10.1002/jobm.201600092

• Saccharomyces cerevisiae
• Candida tropicalis
• Bioremediation
• Glutathione
• Arsenic processing
• Growth optimization

Four arsenic resistant yeast were isolated from the industrial wastewater. Two strains IIB-As1 and IIB-As2 identified as Candida tropicalis and Saccharomyces cerevisiae, respectively. IIB-As1 and IIB-As2 showed maximum arsenic resistance. IIB-As1 showed maximum growth at 35 degrees C whereas it was 30 degrees C for IIB-As2. The yeast isolate showed typical growth curves, but arsenic extended the lag phase. Glutathione plays an important role in metal tolerance. In the present study, As increased the level glutathione and non-protein thiols in yeast isolates. Removal of As from supernatant was analyzed using the atomic absorption spectrophotometer. They removed arsenic from the medium after 72h of incubation. Both yeast strains efficiently removed arsenic from the industrial effluent when used individually or in consortia.

• 出版日期2016-10