Dual Functions of alpha-Ketoglutarate Dehydrogenase E2 in the Krebs Cycle and Mitochondrial DNA Inheritance in Trypanosoma brucei

作者：Sykes Steven E; Hajduk Stephen L^*

来源：Eukaryotic Cell, 2013, 12(1): 78-90.

DOI：10.1128/EC.00269-12

The dihydrolipoyl succinyltransferase (E2) of the multisubunit alpha-ketoglutarate dehydrogenase complex (alpha-KD) is an essential Krebs cycle enzyme commonly found in the matrices of mitochondria. African trypanosomes developmentally regulate mitochondrial carbohydrate metabolism and lack a functional Krebs cycle in the bloodstream of mammals. We found that despite the absence of a functional alpha-KD, bloodstream form (BF) trypanosomes express alpha-KDE2, which localized to the mitochondrial matrix and inner membrane. Furthermore, alpha-KDE2 fractionated with the mitochondrial genome, the kinetoplast DNA (kDNA), in a complex with the flagellum. A role for alpha-KDE2 in kDNA maintenance was revealed in alpha-KDE2 RNA interference (RNAi) knockdowns. Following RNAi induction, bloodstream trypanosomes showed pronounced growth reduction and often failed to equally distribute kDNA to daughter cells, resulting in accumulation of cells devoid of kDNA (dyskinetoplastic) or containing two kinetoplasts. Dyskinetoplastic trypanosomes lacked mitochondrial membrane potential and contained mitochondria of substantially reduced volume. These results indicate that alpha-KDE2 is bifunctional, both as a metabolic enzyme and as a mitochondrial inheritance factor necessary for the distribution of kDNA networks to daughter cells at cytokinesis.

• 出版日期2013-1