A novel molecularly imprinted solid-phase microextraction fiber coupled with high performance liquid chromatography for analysis of trace estrogens in fishery samples

作者：Hu, Yuling; Wang, Yangyang; Chen, Xiaoguang; Hu, Yufei; Li, Gongke^*

来源：Talanta, 2010, 80(5): 2099-2105.

DOI：10.1016/j.talanta.2009.11.015

• Molecularly imprinted
• Solid-phase microextraction
• Estrogens
• Fishery sample

The combination of molecular imprinting and solid-phase microextraction (SPME) technique provides a powerful sample preparation tool in terms of selectivity, simplicity, and flexibility. This paper reports a novel molecularly imprinted polymer (MIP) coated SPME fiber with 17 beta-estradiol as template by improved multiple co-polymerization method The obtained fiber exhibits excellent characteristics such as high porosity, good thermal and chemical stability Extraction performance shows that the MIP-coated fiber has stronger affinity to the template molecule as compared with the commercial SPME fibers and the control polymer-coated fiber without addition of template Owing to the shape and structural compatibility, the obtained fiber also demonstrated specific selectivity to the structural related compounds of 17 beta-estradiol. such as estriol, estrone and 17 alpha-ethynylestradiol. and thus can be applied to simultaneous determination of these estrogens from complex samples coupled with high performance liquid chromatography. The variables that influence extraction were investigated. The MIP-coated fiber demonstrated its efficiency for extraction of estrogens in fishery samples. The detection limits were in the range of 0.98-2.39 mu g L-1, and the recoveries were 80.0-83 6% and 85.0-94.1% for fish and shrimp tissue samples, respectively.

• 出版日期2010-3-15
• 单位中山大学