The formate:oxygen oxidoreductase supercomplex of Escherichia coli aerobic respiratory chain

作者：Sousa Pedro M F; Videira Marco A M; Melo Ana M P^*

来源：FEBS LETTERS, 2013, 587(16): 2559-2564.

DOI：10.1016/j.febslet.2013.06.031

• Formate:oxygen oxidoreductase activity
• Aerobic respiratory chain supercomplex
• Blue native polyacrylamide gel electrophoresis
• Gene transcription
• Bacteria
• Oxygen reductase
• Escherichia coli

The Escherichia con formate:oxygen oxidoreductase supercomplex (FdOx) was investigated with respect to function and composition. Formate oxidoreductase activity was detected in blue native polyacrylamide gel electrophoresis (BN-PAGE) resolved membranes of E. con, which were also capable of cyanide sensitive formate:oxygen oxidoreductase activity. The latter was compromised in strains devoid of specific oxygen reductases, particularly, in those devoid of cytochrome bo(3) or bcI. A principal component analysis (PCA) integrating E. coli aerobic respiratory chain gene transcription, enzyme activity and growth dynamics was performed, correlating formate:oxygen oxidoreductase activity and the transcription of the genes encoding cytochromes bo(3) and bdI, and corroborating previous evidence that associated these complexes in FdOx.

• 出版日期2013-8-19