Development of an Amperometric Hydrogen Peroxide Biosensor based on the Immobilization of Horseradish Peroxidase onto Nickel Ferrite Nanoparticle-Chitosan Composite

作者：Yalciner Furkan; Cevik Emre; Senel Mehmet^*; Baykal Abdulhadi

来源：Nano-Micro Letters, 2011, 3(2): 91-98.

DOI：10.3786/nml.v3i2.p91-98

• Biosensor
• NiFe2O4
• Horseradish peroxidase
• Nanoparticle
• Chitosan

Nickel ferrite (NiFe2O4) nanoparticles have been dispersed in chitosan solution in order to fabricate nanocomposite films. Horseradish peroxidase (HRP) has been immobilized onto this chitosan-NiFe2O4 nanocomposite film via physical adsorption. The size of the NiFe2O4 nanoparticles has been estimated using X-ray diffraction pattern and scanning electron microscopy (SEM) to be 40 +/- 9 nm. The chitosan-NiFe2O4 nanocomposite film and HRP/chitosan-NiFe2O4 bioelectrode have been characterized using SEM technique. The HRP/chitosan-NiFe2O4 nanocomposite bioelectrode has a response time of 4s, linearity as 0.3 to 12mM of H2O2, sensitivity as 22 nA/mM. The effects of pH and the temperature of the immobilized HRP electrode have also been studied.

• 出版日期2011