Use of Commercially Available Bovine Semen Sexing Agent in Buffalo: Preliminary Report of the Effect on the Conception Rate

作者：Barile Vittoria Lucia^*; Pacelli Corrado; De Santis Giuseppe; Baldassi Daniela; Mazzi Marco; Terzano Giuseppina Maria

来源：Buffalo Bulletin, 2013, 32: 502-504.

• buffalo
• AI
• semen sexing
• conception rate

The aim of this work was to test the efficacy in buffalo of a post-thaw semen treatment product, called HeifersPlus (TM) (HP), available on the market for the bovine. This product works by enhancing the fertility of the X-chromosome bearing sperm and slowing the motility of the Y-chromosome bearing one, and attempts to alter the bovine sex ratio in favour of female. In this preliminary report we have evaluated the effect on conception rate (CR) after timed artificial insemination (TAI) comparing the use of HP treated semen with non-treated one in buffaloes synchcronized with Ovsynch; moreover, we have evaluated the effectiveness of delayed insemination, testing the product either at 16h (standard protocol) or 24h (delayed protocol, as suggest by the manufacturer) TAI after GnRH. A total of 281 buffaloes were inseminated, 137 with HP treated semen (treated group) and 144 with no treated semen (control group). Significant differences were found in the CR, being 27.01% in the treated group and 42.36% in the control one (P %26lt;= 0.007). The time of insemination (16h vs 24h) influenced the CR, being reduced at 24h in respect to standard16h TAI. This was particularly evident in the control group where the CR was 50.88 and 36.78%, respectively at 16 and 24 h (P=0.09). In the treated group there was not significant differences in the CR between the two different time of AI (22.81 and 30.00%, respectively at 16 and 24h), however in both time of AI the observed CR was lower compared with the value obtained in the control group with no treated semen.

• 出版日期2013