Expression analysis of integrin beta 1 isoforms during zebrafish embryonic development

作者：Wang, Xin; Li, Liping; Liu, Dong^*

来源：Gene Expression Patterns, 2014, 16(2): 86-92.

DOI：10.1016/j.gep.2014.10.001

• Integrin beta 1
• Expression
• In situ hybridization
• Zebrafish
• Epidermis

Integrins are a superfamily of the major metazoan receptors for cell-cell and cell-extracellular matrix adhesion. Integrins and their ligands play critical roles in a variety of fundamental cellular processes. Integrins are heterodimeric cell surface glycoproteins comprised of non-covalently bound alpha-and beta-subunits. A variety of integrin subunits have been identified in mouse, chicken, zebrafish, Xenopus laevis and other vertebrates. In zebrafish multiple integrin homologs have been identified. However, zebrafish embryo is a largely untapped model for analyzing integrin beta 1 isoforms temporal-spatial expression pattern, function and its relevance to human disease in whole animal level. Currently, we systematically analyzed the expression pattern of zebrafish integrin f31 including integrin beta la (itgbla), integrin beta lb (itgblb), integrin beta lb.1 (itgblb.1), and integrin beta lb.2 (itgblb.2) at embryo stage using whole amount in situ hybridization. We show itgbla, itgblb and itgblb.1 were maternally expressed in zygote, cleavage and blastula periods, while itgbl b.2 was not detectable in the corresponding stages. A more tissue specific pattern emerges during organogenesis, including heart expression for itgb 1 a, myotome borders for itgblb, intestinal epithelium for itgbl b.1, and branchial arch for itgblb.2. All are similarly expressed in the early embryonic epidermis and notochord. Additionally, itgbla, itgblb and itgblb.2 shared the common expression in otic vesicle. Our study provides new insight into the integrin, beta 1 expression and the use of this model organism to tackle future studies on the role of integrin beta 1 in embryo development. 2014 Elsevier B.V.

• 出版日期2014-11
• 单位南通大学