Morphine Preconditioning-induced Endothelial Protection against in vitro Simulated Ischemia-reperfusion Injury

作者：彭龙云; 晏爱珍; 高修仁; 伍贵富; 麦炜颐; 马虹; 左志义

来源：South China Journal of Cardiology, 2009, 10(02): 80-84.

DOI：10.16268/j.cnki.44-1512/r.2009.02.001

• endothelial cells
• morphine
• preconditioning

Objectives To determine whether morphine induced a preconditioning effect in endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were subjected to oxygen-glucose deprivation (OGD) to simulate ischemia in vitro. Cell viability was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Results OGD time-dependently decreased the HUVEC viability. Morphine pretreatment applied 5 min or 24 hr before a 20-hr OGD dose-dependently attenuated the OGD-reduced cell viability. The EC50 for this effect was 1.84 and 0.26 μM, respectively, for morphine applied 5 min or 24 hr before the OGD. Conclusion morphine induces ischemic tolerance in endothelial cells.

• 出版日期2009
• 单位中山大学