Design and performance of an ultra-flexible two-photon microscope for in vivo research

作者：Mayrhofer Johannes M^*; Haiss Florent; Haenni Dominik; Weber Stefan; Zuend Marc; Barrett Matthew J P; Ferrari Kim David; Maechler Philipp; Saab Aiman S; Stobart Jillian L; Wyss Matthias T; Johannssen Helge; Osswald Harald; Palmer Lucy M; Revol Vincent; Schuh Claus Dieter; Urban Claus; Hall Andrew; Larkum Matthew E; Rutz Innerhofer Edith; Zeilhofer Hanns Ulrich; Ziegler Urs; Weber Bruno

来源：Biomedical Optics Express, 2015, 6(11): 4228-4237.

DOI：10.1364/BOE.6.004228

We present a cost-effective in vivo two-photon microscope with a highly flexible frontend for in vivo research. Our design ensures fast and reproducible access to the area of interest, including rotation of imaging plane, and maximizes space for auxiliary experimental equipment in the vicinity of the animal. Mechanical flexibility is achieved with large motorized linear stages that move the objective in the X, Y, and Z directions up to 130 mm. 360 degrees rotation of the frontend (rotational freedom for one axis) is achieved with the combination of a motorized high precision bearing and gearing. Additionally, the modular design of the frontend, based on commercially available optomechanical parts, allows straightforward updates to future scanning technologies. The design exceeds the mobility of previous movable microscope designs while maintaining high optical performance.

• 出版日期2015-11-1