NGAL (Lcn2) monomer is associated with tubulointerstitial damage in chronic kidney disease

作者：Nickolas Thomas L^*; Forster Catherine S; Sise Meghan E; Barasch Nicholas; Sola Del Valle David; Viltard Melanie; Buchen Charles; Kupferman Shlomo; Carnevali Maria Luisa; Bennett Michael; Mattei Silvia; Bovino Achiropita; Argentiero Lucia; Magnano Andrea; Devarajan Prasad; Mori Kiyoshi; Erdjument Bromage Hediye; Tempst Paul; Allegri Landino; Barasch Jonathan

来源：Kidney International, 2012, 82(6): 718-722.

DOI：10.1038/ki.2012.195

• Biomarker
• chronic kidney disease
• pathology

The type and the extent of tissue damage inform the prognosis of chronic kidney disease (CKD), but kidney biopsy is not a routine test. Urinary tests that correlate with specific histological findings might serve as surrogates for the kidney biopsy. We used immunoblots and ARCHITECT-NGAL assays to define the immunoreactivity of urinary neutrophil gelatinase-associated lipocalin (NGAL) in CKD, and we used mass spectroscopy to identify associated proteins. We analyzed kidney biopsies to determine whether specific pathological characteristics associated with the monomeric NGAL species. Advanced CKD urine contained the NGAL monomer as well as novel complexes of NGAL. When these species were separated, we found a significant correlation between the NGAL monomer and glomerular filtration rate (r = -0.53, P %26lt; 0.001), interstitial fibrosis (mild vs. severe disease; mean 54 vs. 167 mu g uNGAL/g Cr, P %26lt; 0.01), and tubular atrophy (mild vs. severe disease; mean 54 vs. 164 mu g uNGAL/g Cr, P %26lt; 0.01). Monospecific assays of the NGAL monomer demonstrated a correlation with histology that typifies progressive, severe CKD.

• 出版日期2012-9