Analysis of cow dung microbiota-A metagenomic approach

作者：Girija D^*; Deepa K; Xavier Francis; Antony Irin; Shidhi P R

来源：Indian Journal of Biotechnology, 2013, 12(3): 372-378.

• Cow dung
• metagenomics
• phylogenetic analysis
• 16S rRNA gene

Cow dung is being used from ancient times in agriculture as it has a significant role in plant growth promotion and plant protection. It is also being used in various religious practices as a purifier. Since only a small fraction of the total microbial diversity can be recovered by culturable methods, a culture independent 16S rDNA approach was taken up for more detailed analysis of cow dung microbiota. Total community DNA was extracted from fresh dung of Brown-Swiss breed and bacterial 16S rRNA genes were subsequently amplified, cloned, sequenced and deposited in GenBank. Bacteria belonging to the phyla Bacteroidetes (38.3%), Firmicutes (29.8%), Proteobacteria (21.3%) and Verrucomicrobia (2%) were identified. Bacteroidetes clones included the genera Bacteroides, Alistipes and Paludibacter; while Clostridium, Ruminococcus, Anaerovorax and Bacillus were predominant in Firmicutes. alpha- and gamma-proteobacterial genera included Acinetobacter, Pseudomonas, Rheinheimera, Stenotrophomonas and Rhodobacter. The Verrucomicrobial clone showed high similarity to Akkermansia. Unculturable bacteria constituted 83.3% in the phylum Bacteroidetes and 87.5% in Firmicutes. All clones under phylum Proteobacteria were culturable bacteria. Eight per cent of the clone library represented previously uncharacterized and unidentified bacteria.

• 出版日期2013-7