Noradrenaline release in rodent tissues is inhibited by interleukin-1 beta but is not affected by urotensin II, MCH, NPW and NPFF

作者：Schulte Kirsten; Kumar Manush; Zajac Jean Marie; Schlicker Eberhard^*

来源：Pharmacological Reports, 2011, 63(1): 102-111.

DOI：10.1016/S1734-1140(11)70404-2

• interleukin-1 beta
• melanin-concentrating hormone
• neuropeptide FF
• neuropeptide W
• noradrenaline release
• superfusion experiments
• urotensin II

We studied whether noradrenaline release is affected by interleukin-1 beta and the neuropeptides urotensin II, melanin-concentrating hormone (MCH), neuropeptide W (NPW) and neuropeptide FF (NPFF). Rodent tissues preincubated with [(3)H]noradrenaline were superfused, and the effect of peptides on the electrically-evoked tritium overflow ("noradrenaline release") was studied. In mouse brain cortex, interleukin-1 beta at 0.3 nM and the prostaglandin E(2) analogue sulprostone at 3 nM inhibited noradrenaline release by about 40%; the effect of interleukin-1 beta developed gradually, whereas the effect of sulprostone occurred promptly. Urotensin II at 0.001-1 mu M did not affect noradrenaline release in rat kidney cortex, whereas 0.01 mu M angiotensin II increased it (positive control). MCH at 0.01-1 mu M did not alter noradrenaline release in the rat brain cortex, and NPW 1 mu M did not affect noradrenaline release in the mouse hypothalamus or hippocampus. In each model, 0.1 mu M sulprostone inhibited noradrenaline release (positive control). NPFF and the NPFF(2) receptor agonist dNPA (1 mu M) did not affect noradrenaline release in the mouse atria; the inhibitory effect of the delta opioid receptor agonist 1 mu M DPDPE on noradrenaline release in this tissue was not altered by NPFF or dNPA at 0.32 mu M but was counteracted by the delta opioid antagonist naltrindole at 0.001 mu M. In conclusion, interleukin-1 beta inhibits noradrenaline release in the mouse cortex; the effect develops gradually, suggesting that it affects protein biosynthesis. Noradrenergic neurons in various tissues from rodents are devoid of presynaptic receptors for urotensin II, MCH, NPW and NPFF. Finally, an interaction between a 5 opioid agonist and NPFF could not be detected.

• 出版日期2011-2