A novel bioassay method: Determining bioactivity of compounds against rice blast (Magnaporthe grisea) with barley plants

作者：Yang X J; Ma X X; Yang L J; Yu D Z; Ni H^*

来源：Journal of Plant Diseases and Protection, 2007, 114(5): 228-231.

• barley
• bioassay method
• fungicide bioactivity
• Magnaporthe grisea
• rice

This paper developed an in vivo bioassay method of determining bioactivity of compounds against Magnaporthe grisea (Hebert) Barr. with barley (Hordeum vulgare L.) plants, instead of rice (Oryza sativa L.) plants. To confirm whether this method could be used to screen active compounds against this fungus or not, experiments were conducted using the five fungicides tricyclazole, kasugamycin, thiophanate-methyl, iprobenfos and salicylic acid with different action modes. The results showed there were close correlation in disease severity and no significant difference in EC50 values between rice and barley, and the coefficients of variations of EC50 values were similar between rice and barley for the four tested fungicides in both detached-leaf-segment and pot-seedling tests. The advantage in bioactivity determination with barley was time-saving. Plant materials could be used at the 1-leaf stage for barley while at the 5-leaf stage for rice. So it shortened about 14 days for the preparation of plant materials for tests.

• 出版日期2007-10
• 单位中国林业科学研究院; 中国农业大学; 湖北省农业科学院