A fast and simple assay for busulfan in serum or plasma by liquid chromatography-tandem mass spectrometry using turbulent flow online extraction technology

作者：Bunch Dustin R; Heideloff Courtney; Ritchie James C; Wang Sihe^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(31): 3255-3258.

DOI：10.1016/j.jchromb.2010.10.003

• Busulfan
• Liquid chromatography
• Tandem mass spectrometry
• Turbulent flow

Busulfan is used in myeloablative preparation regimens for hematopoietic bone marrow transplantation Due to its narrow therapeutic range therapeutic drug monitoring of busulfan is recommended In this study a fast and simple method for measuring busulfan in serum or plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed utilizing turbulent flow online extraction technology Serum or plasma was mixed with acetonitrile containing d(8)-busulfan After centrifugation the supernatant was injected onto a turbulent flow preparatory column then transferred to a C18 analytical column monitored by a tandem mass spectrometer set at positive electrospray ionization The analytical cycle time was 40 min The method was linear from 0 15 to 41 90 mu mol/L with an accuracy of 87 9-103 0% Inter- and intra-assay CVs across four concentration levels were 2 1-7 8% No significant carryover or ion suppression was observed No interference was observed from commercial control materials containing more than 100 compounds Comparison with a well established LC-MS/MS method using patient specimens (n = 45) showed a mean bias 1 3% with Deming regression of slope 1 02 intercept -0 02 mu mol/L. and a linear correlation coefficient 0 9883 The LC-MS/MS method coupled with turbulent flow online sample cleaning tech

• 出版日期2010-12-1